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10. Diva Software Issues . 11. Data Management .
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The HTS supports standard 96 (u bottom plates best) and 384 well plates. It is operated using DiVA allowing users to define customized delivery protocols, mixing, wash and analysis parameters If you have the BD HTS option, connect the HTS sample coupler to the SIT and close the HTS door. Refill fluids and empty waste, if needed, and then select Cytometer > Long Clean . When the long clean is complete, run a fluidics shutdown and turn off the system. BD FACS LSR Fortessa User Manual Flow Cytometry Unit – Biomedical Core facility Technion Faculty of Medicine For help please call: Amir Grau 054-210-0778 Yaakov Sakoury 050-749-8565 Ofer Shenker 052-456-8846 A. Starting Up 1. Turn on the instrument: a. LSR Fortessa - red switch on the wall behind the monitors (Fig.
It runs BD FACS DiVa The BD LSRFortessa™ cell analyzer puts the power of the. BD LSR platform into a flexibility allows users to configure the instrument to meet their Please see the separate filter guide for information on dye HTS Throughput. Acq Instrument: LSR Fortessa HTS. Date:_31 Mar 2015__.
Fortessa FILTERconfig - Lund Stem Cell Center
If you are not familiar with the Windows operating FORTESSA USER GUIDE COMPUTER SETUP: • Turn on computer before turning on the machine • Windows 10 Username: BDOperator • Password: BDIS#1 • Open Tera Term application on desktop and leave running in background • Log in to BD FACSDiva software MACHINE SETUP: • Turn on the machine if you are the first user of the day. There is no Title: Microsoft Word - Stanford Fortessa HTS User Guide.docx Author: lovelace Created Date: 8/14/2013 10:44:41 PM Performance without peer, choice without compromise The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be affordable and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays.
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Log in to Diva at the prompt with your Diva username and password.
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Login to DIVA software. Before proceeding with the HTS, clean the instrument according to standard AMREPFLOW SOPs: • Check fluidics: empty waste and fill sheath. A while back, we were recommended away from routine PRIME functions for this very reason (BD service recommendation). Now, we PRIME only when we see a need – failures or problems with QC, data shifts within an experimental run, or samples fail to flow.
HTS throughput Acquisition: Less than 15 minutes per microtiter plate in high throughput mode using a 2-second
BD FACS LSR Fortessa User Manual Flow Cytometry Unit – Biomedical Core facility Technion Faculty of Medicine For help please call: Amir Grau 054-210-0778 Yaakov Sakoury 050-749-8565 Ofer Shenker 052-456-8846 A. Starting Up 1.
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It processes 96 samples under 15 minutes with less than 0.5% sample carryover. Cited in over 4,500 publications, the BD LSRFortessa is your next step to discovery. This 4.5 days course teaches the operation of the BD LSRFortessa, BD LSRFortessa X-20 and BD FACSCelesta. Additionally, the challenges of successful multi-parameter analysis in terms of antibody-fluorochrome selection, instrument setup including High Throughput Sampler (HTS) & Quality Control (BD™ 2018-12-11 · BD_LSR-Fortessa_18-COLOR_20181211 Cytometer: LSRFortessa Cytometer Name: LSRFortessa Serial Number: H64780000027 Input Device: HTS Window Extension: 10.00 User: Administrator Institution: N/A Software: BD FACSDiva 8.0.1 Date: 12/11/2018 2:59:48 PM Blue Laser (488nm) FSC Bringing boundless potential to multicolor flow cytometry, the BD LSRFortessa X-20 cell analyzer gives you the power to expand and customize your science. Built with innovative optical efficiency and fluidics optimized for sensitivity, this system is backed by BD expert training, service and support—so there is no limit to your research potential. Crafthouse Informed design inspired by the streamlined aesthetic of Deco architecture.
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When the long clean is complete, run a fluidics shutdown and turn off the system. BD Fortessa HTS Procedure: 1) Remove the tube of water from the SIP and connect the HTS sample coupler.
It is operated by users after basic training in operation, maintenance, and troubleshooting. It runs BD FACS DiVa The BD LSRFortessa™ cell analyzer puts the power of the. BD LSR platform into a flexibility allows users to configure the instrument to meet their Please see the separate filter guide for information on dye HTS Throughput. Acq Instrument: LSR Fortessa HTS. Date:_31 Mar 2015__. By:____Teia___.